This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities. Chapters in Biosensors and Biodetection: Methods and Protocols Volume 2, Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors, Second Edition focus on electrochemical biosensors including amperometric, impedance and voltammetric sensors, bioelectronic, piezoelectric, cellular, and molecular biosensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, tips on troubleshooting and avoiding known pitfalls, and step-by-step, readily reproducible laboratory protocols.

Authoritative and practical, Biosensors and Biodetection: Methods and Protocols Volume 2: Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors, Second Edition offers descriptions of major technologies by leading experts in the field in extensive technical detail. The aim of the book is to make biosensors more accessible and understandable to engineers, students, medical professionals, molecular biologists, chemical, and physical science researchers developing biosensor technologies, allowing readers to both understand the technology and to construct similar devices.

1. A Reagentless, Screen¿Printed Amperometric Biosensor for the Determination of Glutamate in Food and Clinical Applications
G. Hughes, R.M. Pemberton, P.R. Fielden, and J.P. Hart
 
2. An Electrochemical DNA Sensing System Using Modified Nanoparticle Probes for Detecting Methicillin-Resistant Staphylococcus aureus
Hiroaki Sakamoto , Yoshihisa Amano, Takenori Satomura , and Shin-ichiro Suye
 
3. Electrochemical Lateral Flow Paper Strip for Oxidative-stress Induced DNA Damage Assessment
Jared Leichner, Mehenur Sarwar, Amirali Nilchian, Xuena Zhu, Hongyun Liu, and Chen-zhong Li
 
4. Application of a Nanostructured Enzymatic Biosensor Based on Fullerene and Gold Nanoparticles to Polyphenols Detection
Cristina Tortolini, Gabriella Sanzò, Riccarda Antiochia, Franco Mazzei, and Gabriele Favero
 
5. Screen-printed All-polymer Aptasensor for Impedance Based Detection of Influenza a Virus
Julie Kirkegaard and Noemi Rozlosnik
 
6. Microfluidic Arrayed Lab-on-a-Chip for Electrochemical Capacitive Detection of DNA Hybridization Events
Hadar Ben-Yoav, Peter H. Dykstra, William E. Bentley, and Reza Ghodssi
 
7. Enzymatic Detection of Traumatic Brain Injury Related Biomarkers
Brittney A. Cardinell and Jeffrey T. La Belle
 
8. Bacterial Detection using Peptide-based Platform and Impedance Spectroscopy
Hashem Etayash, Thomas Thundat, and Kamaljit Kaur
 
9. Fabrication of Lab-on-Paper using Porous Au-paper Electrode: Application to Tumor Marker Electrochemical Immunoassays
Shenguang Ge, Yan Zhang, Mei Yan, Jiadong Huang, and Jinghua Yu
 
10. Electrochemical Biosensors Combined with Isothermal Amplification for Quantitative Detection of Nucleic Acids
Miyuki Tabata, Bo Yao, Ayaka Seichi, Koji Suzuki, and Yuji Miyahara
 
11. A Mini-electrochemical System with Integrated Micropipet Tip and Pencil Graphite Electrode for Measuring Cytotoxicity
Dong-Mei Wu
 
12. All-electrical Graphene DNA Sensor Array
Jeffrey Abbott, Donhee Ham, and Guangyu Xu
 
13. Extended Gate Field-effect Transistor Biosensors for Point-of-Care Testing of Uric Acid
Weihua Guan  and Mark A. Reed
 
14. Highly Sensitive Glucose Sensor Based on Organic Electrochemical Transistor with Modified Gate Electrode
Xudong Ji and Paddy K. L. Chan
 
15. Fabrication of Hydrogenated Diamond Metal-Insulator-Semiconductor Field-Effect Transistors
Jiangwei Liu and Yasuo Koide
 
16. A Light-addressable Potentiometric Sensor for Odorant Detection Using Single Bioengineered Olfactory Sensory Neurons as Sensing Element
Chunsheng Wu, Liping Du, Yulan Tian, Xi Zhang, and Ping Wang
 
17. Piezoelectric Cantilever Biosensors for Label-free, Real-time Detection of DNA and RNA
Alexander P. Haring, Ellen Cesewski and Blake N. Johnson
 
18. Electrochemical Quartz Crystal Nanobalance (EQCN) Based Biosensor for Sensitive Detection of Antibiotic Residues in Milk
Sunil Bhandand Geetesh K. Mishra
 
19. Development of Novel Piezoelectric Biosensor using PZT Ceramic Resonator for Detection of Cancer Markers
Li Su, Chi-Chun Fong, Pik-Yuan Cheung, and Mengsu Yang
 
20. Finger-Powered Electro-Digital-Microfluidics
Cheng Peng and Y. Sungtaek Ju
 
21. Monitoring the Cellular Binding Events with Quartz Crystal Microbalance Biosensors (QCM)
Abdul Rehman and Xiangqun Zeng
 
22. Piezoelectric Plate Sensor (PEPS) for Analysis of Specific KRAS Point Mutations at Low Copy Number in Urine without DNA Isolation or Amplification
Ceyhun E. Kirimli, Wei-Heng Shih, and Wan Y. Shih
 
23. Synthetic Cell-Based Sensors with Programmed Selectivity and Sensitivity
Elvis Bernardaand Baojun Wang
  24. Dynamic Antibiotic Susceptibility Test via a 3D Microfluidic Culture Device Zining Hou, Yu An, and Zhigang Wu
 
25. Aptasensors for Detection of Avian Influenza Virus H5N1
Yanbin Li and Ronghui Wang
26. Optical and Electrochemical Aptasensors for Sensitive Detection of Streptomycin in Blood Serum and Milk
Mohammad Ramezani, Khalil Abnous, and Seyed Mohammad Taghdisi Heydarian
27. A Lateral Flow Biosensor for Detection of Single Nucleotide Polymorphisms
Lingwen Zeng and Zhuo Xiao
 
28. Loop-Mediated Isothermal Amplification and LFD Combination for Detection of Plasmodium falciparum and Plasmodium vivax
Darin Kongkasuriyachai, Suganya Yongkiettrakul, Wansika Kiatpathomchai, and Narong Arunrut
 
29. Characterization of In Vivo Selected Bacteriophage for the Development of Novel Tumor--Targeting Agents with Specific Pharmacokinetics and Imaging Applications
Jessica Newton--Northup and Susan L. Deutscher  
30. Microfluidic `Pouch¿ Chips for Immunoassays  and Nucleic Acid Amplification Tests
Michael G Mauk, Changchun Liu, Xianbo Qiu,  Dafeng Chen,  Jinzhao Song,
And Haim H Bau
 
31. Functionalized Vesicles by Microfluidic Device
Derek Vallejo, Shih-Hui Lee, and Abraham Lee
 
32. Filtration and Analysis of Circulating Cancer Associated Cells from the Blood of Cancer Patients
Cha-Mei Tang, Peixuan Zhu, Shuhong Li, Olga V. Makarova, Platte T Amstutz, and Daniel L. Adams
 
33. Inkjet-Printed Paper Fluidic Devices for Onsite Detection of Antibiotics Using Surface-Enhanced Raman Spectroscopy
Stephen M. Restaino, Adam Berger, and Ian M. White
  34. High Resolution Microultrasound (¿US) Investigation of the Gastrointestinal (GI) Tract Thineskrishna Anbarasan, Christine E. M. Démoré,  Holly Lay, Mohammed R. S. Sunoqrot, Romans Poltarjonoks, Sandy Cochran, and Benjamin F. Cox