This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities. Chapters in Biosensors and Biodetection: Methods and Protocols Volume 2, Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors, Second Edition focus on electrochemical biosensors including amperometric, impedance and voltammetric sensors, bioelectronic, piezoelectric, cellular, and molecular biosensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, tips on troubleshooting and avoiding known pitfalls, and step-by-step, readily reproducible laboratory protocols.

Authoritative and practical, Biosensors and Biodetection: Methods and Protocols Volume 2: Electrochemical, Bioelectronic, Piezoelectric, Cellular and Molecular Biosensors, Second Edition offers descriptions of major technologies by leading experts in the field in extensive technical detail. The aim of the book is to make biosensors more accessible and understandable to engineers, students, medical professionals, molecular biologists, chemical, and physical science researchers developing biosensor technologies, allowing readers to both understand the technology and to construct similar devices.

 A Reagentless, Screen-Printed Amperometric Biosensor for the Determination of Glutamate in Food and Clinical Applications.-  An Electrochemical DNA Sensing System Using Modified Nanoparticle Probes for Detecting Methicillin-Resistant Staphylococcus aureus.-  Electrochemical Lateral Flow Paper Strip for Oxidative-stress Induced DNA Damage Assessment.- Application of a Nanostructured Enzymatic Biosensor Based on Fullerene and Gold Nanoparticles to Polyphenols Detection.- Screen-printed All-polymer Aptasensor for Impedance Based Detection of Influenza a Virus.- Microfluidic Arrayed Lab-on-a-Chip for Electrochemical Capacitive Detection of DNA Hybridization Events.-  Enzymatic Detection of Traumatic Brain Injury Related Biomarkers.- Bacterial Detection using Peptide-based Platform and Impedance Spectroscopy.- Fabrication of Lab-on-Paper using Porous Au-paper Electrode: Application to Tumor Marker Electrochemical Immunoassays.- Electrochemical Biosensors Combined with Isothermal Amplification for Quantitative Detection of Nucleic Acids.- A Mini-electrochemical System with Integrated Micropipet Tip and Pencil Graphite Electrode for Measuring Cytotoxicity.- All-electrical Graphene DNA Sensor Array.- Extended Gate Field-effect Transistor Biosensors for Point-of-Care Testing of Uric Acid.- Highly Sensitive Glucose Sensor Based on Organic Electrochemical Transistor with Modified Gate Electrode.- Fabrication of Hydrogenated Diamond Metal-Insulator-Semiconductor Field-Effect Transistors.- A Light-addressable Potentiometric Sensor for Odorant Detection Using Single Bioengineered Olfactory Sensory Neurons as Sensing Element.- Piezoelectric Cantilever Biosensors for Label-free, Real-time Detection of DNA and RNA.- Electrochemical Quartz Crystal Nanobalance (EQCN) Based Biosensor for Sensitive Detection of Antibiotic Residues in Milk.- Development of Novel Piezoelectric Biosensor using PZT Ceramic Resonator for Detection of Cancer Markers.- Finger-Powered Electro-Digital-Microfluidics.- Monitoring the Cellular Binding Events with Quartz Crystal Microbalance Biosensors (QCM).- Piezoelectric Plate Sensor (PEPS) for Analysis of Specific KRAS Point Mutations at Low Copy Number in Urine without DNA Isolation or Amplification.- Synthetic Cell-Based Sensors with Programmed Selectivity and Sensitivity.- Dynamic Antibiotic Susceptibility Test via a 3D Microfluidic Culture Device.- Aptasensors for Detection of Avian Influenza Virus H5N1.- Optical and Electrochemical Aptasensors for Sensitive Detection of Streptomycin in Blood Serum and Milk.- A Lateral Flow Biosensor for Detection of Single Nucleotide Polymorphisms.- Loop-Mediated Isothermal Amplification and LFD Combination for Detection of Plasmodium falciparum and Plasmodium vivax.- Characterization of In Vivo Selected Bacteriophage forthe Development of Novel Tumor--Targeting Agents with Specific Pharmacokinetics and Imaging Applications.- Microfluidic 'Pouch' Chips for Immunoassays  and Nucleic Acid Amplification Tests.- Functionalized Vesicles by Microfluidic Device.- Filtration and Analysis of Circulating Cancer Associated Cells from the Blood of Cancer Patients.- Inkjet-Printed Paper Fluidic Devices for Onsite Detection of Antibiotics Using Surface-Enhanced Raman Spectroscopy.- High Resolution Microultrasound (¿US) Investigation of the Gastrointestinal (GI) Tract.