This collection of readily reproducible techniques for repairing mammalian DNA contains fourteen entirely new chapters and many extensively revised chapters. The methods presented cover cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting.

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
Malgorzata Z. Zdzienicka

Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes
Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, and Thierry Magnaldo

Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities
William W. Au and Salama A. Salama

Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation
Shruti Nagar, James J. Corcoran, and William F. Morgan

Inhibition of DNA Synthesis by Ionizing Radiation: A Marker for an S-Phase Checkpoint
Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka

Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
George Iliakis, Ya Wang, and Hong Yan Wang

Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage
Xuan Huang and Zbigniew Darzynkiewicz

Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication)
Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi

In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
George Iliakis and Nge Cheong

Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
Michael J. Villalobos, Christopher J. Betti, and Andrew T. M Vaughan

Plasmid-Based Assays for DNA End-Joining In Vitro
George Iliakis, Bustanur Rosidi, Minli Wang, and Huichen Wang

Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants
Rodney S. Nairn and Gerald M. Adair

Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr

Quantitative PCR-Based Measurement of Nuclear and MitochondrialDNA Damage and Repair in Mammalian Cells
Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, and Bennett Van Houten

Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR
Gerd P. Pfeifer

Immunochemical Detection of UV-Induced DNA Damage and Repair
Marcus S. Cooke and Alistair Robson

A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts
Shirley McCready

Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
David L. Mitchell

DNA Damage Quantitation by Alkaline Gel Electrophoresis
Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland

The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Günter Speit and Andreas Hartmann

Fast Micromethod DNA Single-Strand-Break Assay
Heinz C. Schröder, Renato Batel, Heiko Schwertner, Oleksandra Boreiko, and Werner E. G. Müller

32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC
Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki

Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA
Vaughn Smider, Byung Joon Hwang, and Gilbert Chu

Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision
Huixian Wang and John B. Hays

Characterization of Enzymes that Initiate Base Excision Repair at Abasic Sites
Walter A. Deutsch and Vijay Hegde

Base Excision Repair in Mammalian Cells
Yoshihiro Matsumoto

In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti

Biochemical Assays for the Characterization of DNA Helicases
Robert M. Brosh, Jr. and Sudha Sharma

Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell