Part I. Mutant Isolation and Gene Cloning
Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe
Rui G. Martinho and Antony M. Carr
Isolating Mutants of the Nematode Caenorhabditis elegans That Are Hypersensitive to DNA-Damaging Agents
Phil S. Hartman and Naoaki Ishii
Isolating DNA Repair Mutants of Drosophila melanogaster, Daryl S. Henderson
Generation, Identification, and Characterization of Repair-Defective Mutants of Arabidopsis
Anne Britt and Cai-Zhong Jiang
Screening for g-Ray Hypersensitive Mutants of Arabidopsis
Corinne S. Davies
Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
Malgorzata Z. Zdzienicka
Strategies for Cloning Mammalian DNA Repair Genes
Larry H. Thompson
Novel Complementation Assays for DNA Repair-Deficient Cells: Transient and Stable Expression of DNA Repair Genes
Lin Zeng, Alain Sarasin, and Mauro Mezzina
Part II. Recognition and Removal of Inappropriate or Damaged DNA Bases
The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair
Byung Joon Hwang,Vaughn Smider, and Gilbert Chu
Mismatch Repair Assay
Stephanie E. Corrette-Bennett and Robert S. Lahue
Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases
John B. Hays and Peter Hoffman
A Dot Blot Immunoassay for UV Photoproducts
Shirley McCready
Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies
Ann E. Stapleton
Quantification of DNA Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
David L. Mitchell
Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis
John B. Hays and Qishen Pang
DNA Damage Quantitation by Alkaline Gel Electrophoresis
Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland
The Comet Assay (Single-Cell Gel Test): A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Günter Speit and Andreas Hartmann
Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR
Gerd P. Pfeifer and Reinhard Dammann
PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair I: Strand-Specific Quantitative PCR
Keith A. Grimaldi, John P. Bingham, and John A. Hartley
PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair II: Single-Strand Ligation-PCR
Keith A. Grimaldi, Simon R. McAdam, and John A. Hartley
Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
R. Michael Anson and Vilhelm A. Bohr
Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA
Walter A. Deutsch and Adly Yacoub
Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts
Yoshihiro Matsumoto
In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
Guido Frosina, Enrico Cappelli, Paola Fortini, and Eugenia Dogliotti
Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
Johnson M. S. Wong, Zhigang He, and C. James Ingles
In Vitro Excision Repair Assay in Schizosaccharomyces pombe
Bernard Salles and Patrick Calsou
Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines
Kenji Kohno and Takuya Shimamoto
Nucleotide Excision Repair in Nuclear Extracts from Xenopus Oocytes
Eric J. Ackerman, Lilia K. Koriazova, Jitendra K. Saxena, and Alexander Y. Spoonde
Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA
Maureen Biggerstaff and Richard D. Wood
Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site
Mahmud K.