The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid.

Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.

Manipulation and Detection of Survival Signals.- Functional Analysis of the Antimitogenic Activity of Tumor Suppressors.- Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination.- Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells.- The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress.- Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression.- Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells.- TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s).- Manipulation and Edtection of Oncogenic Signals.- Kinetworks(TM) Protein Kinase Multiblot Analysis.- Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers.- Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay.- Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay.- Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells.- Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD).- Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model.- Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer.- Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes.- Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes.- Protein Interactions.- Methods for the Study ofProtein-Protein Interactions in Cancer Cell Biology.- Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay.- Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions.- Genomic Rearrangements.- Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing.- Clonality Analysis by T-Cell Receptor ? PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL).